A new semi-defined medium for Trypanosoma brucei sspp.

Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media.

Two simple media for biochemical experimentation with cultured procyclic Trypanosoma brucei.

Two simple media are presented which are particularly well suited for biochemical experimentation with procyclic culture forms of Trypanosoma brucei. ME-83 is a simple semi-defined medium which supports active cell growth, and from which individual components can conveniently be deleted or replaced. HHP-84 is a fully defined minimal medium, which allows vigorous cell motility over extended time...

Establishment of ATP-Based Luciferase Viability Assay in 96-Well Plate for Trypanosoma congolense

Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HT...

Evaluation of the in vitro transformation technique to distinguish Trypanosoma evansi from cyclically transmitted Trypanozoon stocks.

In order to initiate transformation into procyclic forms, bloodstream trypanosomes, were transferred to semi-defined medium at 27 degrees C. All stocks previously classified as Trypanosoma brucei rhodesiense, T.b. gambiense and T.b. brucei transformed into procyclic forms. None of the 31 characterized T. evansi stocks transformed into procyclic forms, but died between day 4 and day 6 in culture...

Efficacy of repeated doses of diminazene aceturate (Dinazene®) in the treatment of experimental Trypanosoma brucei infection of Albino rats

The efficacy of repeated doses of Dinazene® in Albino rats experimentally infected with Trypanosoma brucei (Gboko strain) was investigated. A total of 30 adult female Albino rats weighing 130-190 g were used for the study. They were assigned to six groups (groups A-F) of five rats each. Groups A-D were infected intraperitoneally with 1.0 × 106 trypanosomes in 400 μL of PBS diluted blood while g...